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dc.contributor.authorBondžić, Aleksandra
dc.contributor.authorJovanović, Dunja
dc.contributor.authorArsenijević, Nevena
dc.contributor.authorLaban, Bojana
dc.contributor.authorLazarević-Pašti, Tamara
dc.contributor.authorKlekota, Urszula
dc.contributor.authorBondžić, Bojan
dc.date.accessioned2023-04-21T06:32:29Z
dc.date.available2023-04-21T06:32:29Z
dc.date.issued2022-08-11
dc.identifier.citationMinistry for Education, Science and Technological Development of the Republic of Serbia, grant numbers: 451-03-68/2022-14/200017en_US
dc.identifier.urihttps://platon.pr.ac.rs/handle/123456789/1242
dc.description.abstractThe study of the interactions between nanoparticles (NPs) and proteins has had a pivotal role in facilitating the understanding of biological effects and safe application of NPs after exposure to the physiological environment. Herein, for the first time, the interaction between L-methionine capped silver nanoparticles (AgMet), and bovine serum albumin (BSA) is investigated in order to predict the fate of AgMet after its contact with the most abundant blood transport protein. The detailed insights into the mechanism of interaction were achieved using different physicochemical techniques. The UV/Vis, TEM, and DLS were used for the characterization of the newly formed “entity”, while the kinetic and thermodynamic parameters were utilized to describe the adsorption process. Additionally, the fluorescence quenching and synchronous fluorescence studies enabled the prediction of the binding affinity and gave us insight into the influence of the adsorption on the conformation state of the BSA. According to the best of our knowledge, for the first time, we show that BSA can be used as an external stabilizer agent which is able to induce the peptization of previously agglomerated AgMet. We believe that the obtained results could contribute to further improvement of AgNPs’ performances as well as to the understanding of their in vivo behavior, which could contribute to their potential use in preclinical research studies.en_US
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.title“Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interactionen_US
dc.title.alternativeInternational Journal of Molecular Sciencesen_US
dc.typeclanak-u-casopisuen_US
dc.description.versionpublishedVersionen_US
dc.identifier.doihttps://doi.org/10.3390/ijms23168985
dc.citation.volume23
dc.citation.issue16
dc.citation.spage8985
dc.subject.keywordsprotein coronaen_US
dc.subject.keywordsAgNPsen_US
dc.subject.keywordsBSAen_US
dc.subject.keywordsadsorptionen_US
dc.subject.keywordskineticsen_US
dc.subject.keywordsthermodynamic studyen_US
dc.type.mCategoryM21en_US
dc.type.mCategoryopenAccessen_US
dc.type.mCategoryM21en_US
dc.type.mCategoryopenAccessen_US


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